Anil plant is native to sub-tropical and tropical Americas, including the southern United States, the Caribbean, Mexico, Central America, and South America as far south as northern Argentina. This species has been widely introduced to other parts of the world and today has a pantropical distribution. In experiments with livestock, several species of Indigofera have proven toxic, and have caused various symptoms including muscular spasms, paralysis, general weakness and even death. This study focusses on establishing the toxicity profile of the plant extract. The plant extract will be tested for acute and chronic toxicity as per OECD guidelines. The present investigation demonstrates that at doses consumed in the traditional medicine, the ethanol extract of Indigofera suffruticosa L. may be considered as relatively safe, as it did not cause either any lethality or changes of in the general behavior in both the acute and chronic toxicity studies in rats.